An Immunochemical Approach to Quantify and Assess the Potential Value of the Pseudomonas Quinolone Signal as a Biomarker of Infection

Quorum sensing (QS) is a bacterial cell density-based communication system using low molecular weight signals called autoinducers (AIs). Identification and quantification of these molecules could provide valuable information related to the stage colonization or infection as well disease. With this scenario, we report here for first time development antibodies against PQS (pseudomonas quinolone signal), main signaling molecule from pqs QS Pseudomonas aeruginosa, microplate-based enzyme-linked immunosorbent assay (ELISA) able quantifying in complex biological media nanometer range (LOD, 0.36 ± 0.14 nM culture broth media). Moreover, ELISA reported has been found be robust reliable, providing accurate results media. The technique allowed us follow up profile release clinical isolates obtained patients different disease status. A clear correlation was between immunoreactivity equivalents chronic acute conditions, which supports differences on virulence behavior strains due their adaptation capability host environment. point potential biomarker value technology developed improving diagnosis management P. aeruginosa infections based precise identification pathogen, appropriate stratification according status, knowledge progression.